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. 2005 Aug;79(15):9970–9981. doi: 10.1128/JVI.79.15.9970-9981.2005

FIG. 3.

FIG. 3.

(a) Western blots developed with anti HSV-1 R1 antiserum 106. In duplicate, whole-cell extracts were prepared from Vero (lanes 1, 2, 7, 8), CHO (lanes 3, 4, 9, 10) or mouse melanoma (lanes 5, 6, 11, 12) cells infected with 5 PFU/cell HSV1716gfp/Vero (lanes 1 to 6) or HSV1716gfp/BHK (lanes 7 to 12). For panels b to f, Western blots developed with monoclonal antibodies against ICP4 (b), ICP0 (c), VP16 (d), gB (e), and gC (f) are shown. Whole-cell extracts were prepared from CHO (lanes 1 and 2) or BHK (lane 3) cells infected with 10 or 5 PFU/cell HSV1716gfp/Vero (lane 1 and 3, respectively) or 10 PFU/cell HSV1716gfp/BHK (lanes 2).