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. 2005 Aug;79(15):9970–9981. doi: 10.1128/JVI.79.15.9970-9981.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 4. — (a) Electron micrograph of CHO cells 24 h after infection with 5 PFU/cell HSV1716gfp/BHK. Examples of capsids in the nucleus (Nuc) are indicated by a black arrow, enveloped virions in the cytoplasm (Cyt) are indicated by a white arrow, and unenveloped capsids in the cytoplasm are indicated by a white arrowhead. Bar, 1 μm. For panels b to f, unprocessed images (magnification, ×200) from fluorescent microscopy are shown. (b) BHK cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in CHO cells; (c) BHK cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in Vero cells; (d) C8161 cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in CHO cells; (e) BHK cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in C8161 cells; (f) CHO cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in CHO cells. For panels g, h, and i, unprocessed images (magnification, ×100) from fluorescent microscopy are shown for CHO (g), BHK (h), and Vero (i) cell lines 5 days after infection with 1 × 10³ PFU HSV1716gfp/BHK (g, h) or HSV1716gfp/CHO (i).

FIG. 4. — (a) Electron micrograph of CHO cells 24 h after infection with 5 PFU/cell HSV1716gfp/BHK. Examples of capsids in the nucleus (Nuc) are indicated by a black arrow, enveloped virions in the cytoplasm (Cyt) are indicated by a white arrow, and unenveloped capsids in the cytoplasm are indicated by a white arrowhead. Bar, 1 μm. For panels b to f, unprocessed images (magnification, ×200) from fluorescent microscopy are shown. (b) BHK cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in CHO cells; (c) BHK cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in Vero cells; (d) C8161 cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in CHO cells; (e) BHK cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in C8161 cells; (f) CHO cells after 8 h of infection with 5 PFU/cell HSV1716gfp propagated in CHO cells. For panels g, h, and i, unprocessed images (magnification, ×100) from fluorescent microscopy are shown for CHO (g), BHK (h), and Vero (i) cell lines 5 days after infection with 1 × 10³ PFU HSV1716gfp/BHK (g, h) or HSV1716gfp/CHO (i).