FIG. 5.

Detection of viral transcripts by RT-PCR in HPDA infected with HHV-6A or HHV-6B. RT-PCR for U33, U12, U39, U27, and U94 at day 3 postinfection is shown. Each set of primers was tested on HPDA cDNA (S), nonretrotranscribed RNA as a negative control (C−), and a positive control of cDNA obtained from SupT-1 cultures infected with either viral variant (C+). (A) HPDA infected with U1102. (B) HPDA at day 3 postinfection with HHV-6B strain Z29. (C) After the third passage, no viral transcripts were detected in HPDA infected with Z29. β-actin was used as an internal control.