TABLE 1.

Primers used in RT-PCR^a

Coding ORF	Functionb	Primers	cDNA length (bp)	DNA length (bp)
U33	Capsid protein	Primary F, GACAGCGCTGACACTGAAAA	174	174
		Primary R, CGGTGATCGGAACAAATTCT
		Nested F, CGGTCACGTTGTAGAGATGC	117	117
		Nested R, CGGTGATCGGAACAAATTCT
U12	G-protein-coupled receptor	Primary F, CACTGTCATTGAGCTGTCCAA	238	327
		Primary R, ACCACATGAGCACAAAATCG
		Nested F, CACTGTCATTGAGCTGTCCAA	113	202
		Nested R, TGAGCGTGATTCCGGTAACT
U39	Glycoprotein B	Nested F, CCATACCCTCCTCCTTTTCC	129	129
		Nested R, CTTTCCTTATGCCACCAATCC
U27	DNA polymerase processivity factor	Primary F, CGGGAACATAGAGAAACGAGAG	209	209
		Primary R, GACAAAAACAAACATTCCGCC
		Nested F, CTACAGTGACTTTCACGCC	100	100
		Nested R, GACAAAAACAAACATTCCGCC
U94	Transactivation, AAV-2	Primary F, GCATACGTGCACCAATCATC	168	168
		Primary R, ACGCTCAAGCGGAGAATAAA
		Nested F, GCATACGTGCACCAATCATC	100	100
		Nested R, GCGCAACGATAGGAAAACAC
Human β-actin		Primary F, CAAGAGATGGCCACGGCTGCT	275	340
		Primary R, TCCTTCTGCATCCTGTCGGCA

^aORF, open reading frame; AAV-2, adeno-associated virus type 2.

^bSee reference 16.