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. 2005 Aug;79(15):9735–9745. doi: 10.1128/JVI.79.15.9735-9745.2005

FIG. 1.

FIG. 1.

Characterization of an inducible VP22-expressing 293 cell line. (A) The VP22-293-EcR cell line was induced to express VP22 with 1 μg/ml muristerone A, and samples were harvested at various times after induction and analyzed by SDS-PAGE and Western blotting with antibodies against VP22 and α-tubulin. (B) VP22-293-EcR cells grown on coverslips were left uninduced or induced with muristerone A, fixed 24 h later, and stained with polyclonal anti-VP22. Arrows indicate cells with VP22 in the nucleus; arrowheads indicate cells with VP22 in the cytoplasm. -ve, parental 293-EcR cell line.