FIG. 4.

The Δ22 virus exhibits an early delay in the infectious process. (A) Monolayers of Vero cells were infected with either WT or Δ22 virus stocks at a multiplicity of 10 followed by pulse-labeling with [³⁵S]methionine at various times after infection. The cells were harvested and analyzed by SDS-PAGE and autoradiography. Asterisks indicate virus-induced proteins. Numbers on the left indicate molecular masses in kilodaltons. (B) Monolayers of Vero cells were infected with the same WT or Δ22 virus stocks at a multiplicity of 10, and samples were harvested at the indicated times after infection and analyzed by SDS-PAGE and Western blotting with antibodies against virus proteins as indicated. (C) Monolayers of Vero cells were infected with WT, Δ22, or Δ22R virus at a multiplicity of 10, and samples were harvested at the indicated times after infection and analyzed by SDS-PAGE and Western blotting with antibodies against VP22 and ICP0. h.p.i., hours postinfection.