FIG. 7.

VP22 colocalizes with ICP0 and is required for ICP0 incorporation into virions. (A) Vero, MDBK, and BHK cells grown on coverslips were infected with the GFP-VP22-expressing virus 166v at a multiplicity of 10. At 6 h, the cells were fixed and processed for immunofluorescence with the anti-ICP0 antibody 11060. The insets show magnified images of the indicated regions. (B) Virion samples of WT, Δ22, and Δ22R viruses grown in BHK21 cells were analyzed by SDS-PAGE followed by Western blotting with antibodies against VP5, ICP0, ICP4, and VP22.