FIG. 8.

Adoptive transfer of Ad35-specific CD8⁺ T lymphocytes. Donor mice were immunized twice with 10¹⁰ vp rAd35-Empty or saline control. CD8⁺ T lymphocytes were purified from splenocytes, and 5 × 10⁷ cells were adoptively transferred to naïve recipient mice prior to vaccination with (A and B) 2 × 10⁸ vp rAd35-Gag or (C and D) 2 × 10⁸ vp rAd11-Gag. Gag-specific cellular immune responses were assessed by (A and C) D^b/AL11 tetramer binding assays and (B and D) IFN-γ ELISPOT assays using pooled Gag peptides and individual AL11 and KV9 epitope peptides.