TABLE 2.
TGMV AL3 mutantsa
| Mutant | Mutated residue(s) | Mutagenesis primer | Expression cassette
|
|
|---|---|---|---|---|
| Plant | Insect | |||
| t29 | YFK30 | CAAATCCCTCgcggcCgcGATAATCAGCG | pNSB724 | pNSB751 |
| t53 | QIRFNHN54 | ATATACCACTTAGCaGCcGCgGCCGCcGCCGCCTGAGGAGAGC | pNSB798 | pNSB752 |
| t69 | F66, NF69, WT73 | CCACAAGGCAgcTCTcgcagtaCAAGTAggtgCtACATCGACGA | pNSB799 | pNSB753 |
| t86 | NRFK87 | ACTTATTTAAATgcggccgcATACTTAGTGATG | pNSB800 | pNSB804 |
| t93 | VMLYLE95 | TTAAATACTTAGcGcGgcCgcTGctCAGTTAGGC | pNSB801 | pNSB805 |
a
Lowercase bases indicate altered nucleotides in the mutant.