FIG. 2.
Increased MMP-9 transcripts in EBNA3C and Nm23-H1 C33a stable cell lines as detected by real-time RT-PCR analysis. Total RNA was isolated from 10 million C33a cells by Trizol reagent. Five micrograms of RNA was used in the Superscript First Strand synthesis system to construct the cDNA. Real-time PCR was performed using the DyNAmo SYBR green quantitative PCR kit with β-actin as the standard. The PCR data are expressed as n-fold activation relative to normal C33a cells as calculated by the ΔΔCt method. A series of three independent experiments was used to calculate the mean and standard deviation. WB, Western blotting.