FIG. 5.

(A to D) Both EBNA3C and Nm23-H1 coimmunoprecipitate with transcription factors shown to be important for the regulation of MMP-9. Thirty million 293T cells were transfected with EBNA3C, Nm23-H1, or both expression vectors. At 20 h posttransfection, the cells were harvested and lysed with RIPA buffer; the lysates were then used for the IP experiments. Mouse monoclonal antibodies against Sp1, rabbit monoclonal antibody against c-Jun, and rabbit polyclonal antibodies against c-Fos and NFκB p50 were used to show that either EBNA3C or Nm23-H1 immunoprecipitated with the transcription factor. L, lysate; PC, preclear; WB, Western blot.