FIG. 5.
Comparison of complete 3Cpro digestion products of TBP, ΔN100 TBP, and ΔC239 TBP. (A) In vitro translated [35S]methionine-labeled wt TBP (lanes 1 and 2), ΔN100 (lanes 3 and 4), and ΔC239 (lanes 5 and 6) were digested with buffer (lanes 1, 3, and 5) or 3Cpro (lanes 2, 4, and 6) to completion. The radiolabeled products were analyzed by SDS-PAGE. (B) A schematic presentation of the predicted 3Cpro cleavage products generated from TBP and truncated TBP molecules. Arrowheads indicate Q18-G19 and Q104-S105 cleavage sites. The numbers within parentheses indicate the molecular weights of the products, while the numbers marked with asterisks indicate actual molecular weights of these products compared to how they migrate during gel electrophoresis.