FIG. 7.

ΔN100 TBP is highly defective in restoring RNA Pol II-mediated transcription in PV-infected extracts. (A) TATA box-dependent in vitro transcription from a template DNA containing Ad MLP was performed in poliovirus-infected (4 h postinfection) cell extracts in the absence and presence of purified ΔN100 TBP or wt TBP. The primer-extended product was quantified by densitometric scanning, and the percentage of relative transcription with respect to 4-h mock-infected control was plotted against the concentration of TBP in the reaction. (B) Western blots of various concentrations of wt and ΔN100 TBP used in the transcription assay are shown.