FIG. 2.

Infiltration and targeting of reconstituted MCMV-immune lymphocytes in the brain. PHK-26-labeled lymphocytes (red) were visualized following systemic reconstitution in SCID mice. Both VSV- and MCMV-immune lymphocytes penetrated the blood-brain barrier and surveyed brain parenchyma, but MCMV antigen specificity was required for recognition and elimination of MCMV infection (green). A to D) Brain of representative SCID mouse following reconstitution with VSV-immune cells; note scattered red lymphocytes throughout the parenchyma (arrows). E and F) Brain of representative SCID mouse following reconstitution with MCMV-immune cells. G) Repopulated spleen of SCID mouse, showing transferred red lymphocytes. A, C, E, and G) Red PHK-26-labeled lymphocytes; B, D, and F) green MCMV-GFP. PKH-26 and MCMV-GFP images are of the same field. Bar: 500 μm in A; 100 μm in G; 50 μm in F.