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. 2005 Aug;79(15):9527–9539. doi: 10.1128/JVI.79.15.9527-9539.2005

FIG. 5.

FIG. 5.

Flow cytometric verification of sorting efficiency. Scatter plots selected by cellular phenotype confirm cell-sorting effectiveness for adoptive reconstitution of SCID mice. (A) Unsorted splenocytes versus T-cell-enriched splenocytes following panning, <5% remaining; B lymphocytes (B220+ cells); (B) >95% pure isolation by negative selection of CD4 or CD8+ T cells using magnetic bead separation.