FIG. 2.

Yeast two-hybrid analysis of the interaction of UL36 and UL37. (A) Summary of full-length UL37 binding to fragments of UL36. The region 453 to 608, which is homologous to the previously determined UL37-binding site in PRV, is indicated (20). (B) Summary of quantitative liquid β-galactosidase assay for UL36 fragments 124 to 511 and 512 to 767. The activity was calculated from the following equation: β-galactosidase activity = 1,000 × A₄₂₀/(t × V × OD₆₆₀), where t is time (in minutes) of incubation, V is the volume of cells (in ml) used in the assay, and OD₆₆₀ is the optical density at 660 nm. The values obtained for β-galactosidase activity are the averages of measurements from at least three separate colonies. X-gal, 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside; −, no growth or color change at day 3; +++, darkest blue colonies at day 3.