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. 2005 Aug;79(15):9566–9571. doi: 10.1128/JVI.79.15.9566-9571.2005

FIG. 3.

FIG. 3.

In vitro pull-down assay to assess the interaction of UL46 and UL48. Bacterial lysates containing recombinant untagged UL48 were added to GST or GST-UL46 on glutathione-Sepharose beads. Protein complexes were subsequently eluted and separated by SDS-PAGE (4 to 20%). (A) Immunoblotting with anti-UL48 confirms the presence of recombinant untagged UL48 coeluting preferentially with GST-UL46 in the absence of other viral proteins. (B) Coomassie blue staining to confirm the presence of GST proteins eluted from glutathione-Sepharose beads. The predicted masses of various protein products are as follows: GST, 26 kDa; GST-UL46, 104 kDa; untagged UL48, 54 kDa.