FIG. 4.

Coimmunoprecipitation assay. HeLa cells were cotransfected with expression plasmids encoding myc-tagged and HA-tagged proteins. Cells lysates were harvested at 48 h posttransfection and immunoprecipitated (IP) with mouse anti-myc monoclonal antibody. Protein complexes were separated by SDS-PAGE (4 to 20%). (A) Immunoblotting with mouse anti-myc monoclonal antibody; (B) immunoblotting with rabbit anti-HA polyclonal antibody. The positions of UL36, UL37, and KIF5B along with coeluting mouse anti-myc IgG heavy and light chains detected by the secondary anti-mouse conjugate used during immunoblotting are indicated. The predicted masses of various protein products are as follows: UL36 512 to 767, 30 kDa; UL37, 120 kDa; KIF5B 814 to 963, 19 kDa.