FIG. 4.

Expression profile of CcBV cystatin genes in parasitized Manduca sexta by Northern blot analysis (A) Time course experiment using RNA extracted from nonparasitized (NP) or parasitized Manduca sexta fat body at different time points postparasitization as indicated. (B) Tissue-specific expression using RNA extracted from nonparasitized (NP) or parasitized Manduca sexta at different time points postparasitization as indicated. A total of 20 μg of RNA was loaded in each lane. Cystatin 1 cDNA was used as a probe. As a control, blots were hybridized with Manduca sexta actin. Levels of cystatin expression are indicated relative to that of the control actin. The values correspond to the ratios obtained after Instant Imager scanning of the Northern blots presented.