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. 2005 Aug;79(15):9388–9396. doi: 10.1128/JVI.79.15.9388-9396.2005

FIG. 6.

FIG. 6.

Identification of DAP10, DAP12, and NKG2D-S mRNA in CNS-derived γδ T cells. NKG2D+ γδ T cells from the CNS of JHM-infected TCRβ−/− mice or NKG2D+ CD8 T cells from an uninfected C57BL/6 spleen were sorted as described in Materials and Methods. (A) mRNA was extracted from the sorted cells and subjected to RT-PCR for DAP10, DAP12, and hypoxanthine phosphoribosyltransferase (HPRT). Lanes 1 to 3, CNS-derived γδ T cells from three individual JHM-infected TCRβ−/− mice. Lane 4, splenic NKG2D+ CD8 T cells from an uninfected C57BL/6 mouse. Lane 5, negative control. (B) mRNA was subjected to RT-PCR for NKG2D-S. Lanes 1 and 2, CNS-derived γδ T cells from two individual JHM-infected mice. Lane 3, negative control.