FIG. 1.

Schematic diagram of eukaryotic expression vectors. All constructs (not drawn to scale) were based on pSINCP. The pMSIN-H vector contains a full-length MV-H under the control of Sindbis virus subgenomic promoter (SubP). pMSIN-FL and pMSIN-FdU encode MV-F with or without its 5′ NTR, respectively. The bicistronic expression vectors, pMSINH-FdU and pMSINH-IRESFdU, encode MV-F under the control of SubP or the IRES element, respectively. The plasmid pMSINH-FdU(dC) contains MVF with mutations in its cleavage site (RRHKR to RRTSR at amino acids 108 to 112). The fusion construct, pMSINH-FdU(fu), encodes an in-frame fusion of MV-H, lacking its termination codon, with MV-F, lacking its signal sequence. Numbers in parentheses indicate the number of amino acids encoded by each DNA fragment.