FIG. 7.
Measles virus-specific IFN-γ responses. On day 70, mice were sacrificed, their spleens were extracted and homogenized, and splenocytes were isolated. Single-cell suspensions were pooled within each group and restimulated in vitro for 48 h with 5 μg/ml MV lysate in an IFN-γ-specific ELISPOT assay. Results are expressed as the mean IFN-γ spot-forming cells (SFC) ± SD from triplicate wells/106 cells. IFN-γ spots in wells containing medium (controls) were subtracted from the spots counted in wells containing MV for all experimental groups.