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. 2005 Jul 12;6:173. doi: 10.1186/1471-2105-6-173

Table 4.

Results for the Like-like data

Method Estimated False Positive Rate
Rule of Two (on unnormalized data) 42.1%
Rule of Two (on mean loess normalised data) 3.7%
Rule of Two (on mean and variance loess normalised data) 1.4%
NUDGE 0.4%

SAM, EBarrays and t tests are not applicable to single slide data.