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. 2005 Jun 20;5:19. doi: 10.1186/1472-6750-5-19

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Copyright © 2005 Lewin et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of hemolysin genes in E. coli directed by viral promoters. Hemolysis of sheep blood erythrocytes by E. coli DH5α containing the vlly coding sequence without promoter (panels A and D), with the promoter from the polyhedrin gene (P PH: panels B and D) or with the immediate early promoter from CMV (P CMV: panels C and D). Panels A to C show the hemolysis in blood agar plates visible as cleared zones around the colonies. Panel D indicates the percentage of erythrocytes lysed in a liquid blood assay. The columns show the average of two measurements.