Table 1.
Comparison of Jagged1-Induced RBP/JK Activity in Cells Expressing either Wild-Type Notch3, Mutant Notch3, or Simulated Heterozygous Notch3 Mutants
| Type of Cell | % of Relative Activity Induced by Jagged1a | P Values versus WT-Notch3 |
| WT | 100 | … |
| R90C | 79.7 ± 13.8 | NSb |
| R90C + WT | 99.0 ± 23.8 | NSb |
| C428S | 18.0 ± 11.2 | <.001 |
| C428S + WT | 59.7 ± 10.6 | <.01 |
| C542Y | 30.0 ± 6.8 | <.001 |
| C542Y + WT | 62.3 ± 11.7 | <.01 |
a
Jagged1-induced activation was expressed as the ratio of normalized luciferase value induced by Jagged1-expressing cells compared with that obtained with parental L cells; relative activity reflects activation induced by Jagged1, relative to that obtained with wild-type Notch3. The mean ± SEM data from three independent experiments performed in triplicate are presented.
b
NS = not significant.