Table 1.

PCR Primers for Amplification, Restriction Analysis, and Sequencing of the CARD15 Mutations and Polymorphisms

		Primera		PCR or RestrictionFragment Size(bp)
Mutation	Restriction Enzyme	Forward	Reverse	Wild Type	Mutant
R702W	MspI	CTTCCTGGCAGGGCTGTTGTC	CATGCACGCTCTTGGCCTCAC	76+54+24+22	130+24+22
G908Rb	HhaIb	AAGTCTGTAATGTAAAGCCACb	CCCAGCTCCTCCCTCTTCb	380	242+138
1007fs	NlaIV	CCTGCAGTCTCTTTAACTGG	CTTACCAGACTTCCAGGATG	168	128+40
P268S	BamHI	TGCCTCTTCTTCTGCCTTCCb	AGTAGAGTCCGCACAGAGAGb	422	247+175
M863V	NcoI	CTGTTTGCATGATGGGGGGb	GGGAGATCACAGCATTAGAGb	212+160	372
N852S	AluI	CTGTTTGCATGATGGGGGGb	CAGCCGTCAGTCAATTTGTAG	151	129+22
IVS8+158	XhoI	TGCAGTTTTCTTGGGGAGAT	TGTACCTGATCCAGCCCAAT	125+95	220
L248R	MspI	TGCCTCTTCTTCTGCCTTCCb	AGTAGAGTCCGCACAGAGAGb	44+375	44+117+258
A612T	XcmI	ATGTGCTGCTACGTGTTCTCb	CAGACACCAGCGGGCACAGb	456	155+301
Sequencing primers for promoter region		TGTGGTCTCAGGAAGGAAGG	ACTGGTGAGCCTGGATCTGT	918	…
		Wild Type	Mutant
D113Nc		AGCCCAGGCCGACAGCCA	AGCCCAGGCCAACAGCCA	…	…
I363Fc		GTTGGTCAAGAAGACATCTTC	GTTGGTCAAGAAGACTTCTTC	…	…
L550Vc		ACCCTCCTGCACCTGGGCA	ACCCTCCTGCACGTGGGCA	…	…

^aModified (or, in the case of dot-blot probes, mutated) bases are underlined.

^bPCR primers and/or conditions according to or modified from Lesage et al. (2002).

^cDot-blot probe.