Figure 4.

Alignment of predicted HSN2 peptide sequences for vertebrate orthologs. a, The conserved HSN2 ORF was identified from the genomic assemblies of human, mouse, rat, and zebrafish (Zfish) and from the pig cDNA clone. BLAST searches were done through the NCBI Web site. Genomic sequences for HSN2 orthologs were identified within GenBank sequence files AC004765 (human), AC106932 and AC106348 (rat), AC113092 (mouse), and BX321885 (zebrafish). Prosite searches were done through the EBI server. For the predicted peptide sequence alignment, sequences were aligned using Clustal 1.8, available from the BCM Search Launcher Web site. Aligned sequences then were shaded using BOXSHADE. Functional bioinformatics analysis was performed using SignalP. Conserved residues are shaded. Residues are numbered from the most upstream start ATG. Asterisks (*) above N-terminal residues indicate potential initiating methionines; asterisks at the C-termini indicate the stop codons. The signal peptide and predicted cleavage residue are indicated at the N-terminus. Positions of causative mutations in human populations are indicated. b, Alignment of 3′ ends of resequenced human and pig cDNA clones with the human genomic sequence, showing conservation of polyadenylation sites. The putative polyadenylation signal is shown above the aligned sequences.