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Granulosa cell-growth assay evaluated by direct cell count (see methods in appendix A [online only]). Results (mean ± SD) of a representative assay using different doses of BMP15-Myc-His fusion proteins. Tagged proteins were purified on nickel columns, and doses of purified proteins (0.1 or 0.2 μg/ml) were tested in triplicate wells. An asterisk (*) indicates P<.05 versus control and mutant preparations (two-tailed, unpaired Student’s t test).
