Table A.
Candidate-Gene Primers
|
Primer Pair |
|||||
| Forward |
Reverse |
||||
| CandidateGene | Name | Sequence | Name | Sequence | Product Size(bp) |
| EVX2 | EVX2-1F | GCTGAATGAGAATTAGCCCTAGGC | EVX2-1R | AAGAGGATGGGACTGGAGAGCG | 552 |
| EVX2-2F | CTGTTGGTCCTACAGCCACTCATG | EVX2-2R | AAACAATCAACCCAGATGCCC | 379 | |
| EVX2-3F | TGGGACAGGAGCTGGGAACA | EVX2-3R | AACTGAGGCACGAGCAGGGT | 539 | |
| EVX2-4F | TGTGTAGCTTCCGCCACCCT | EVX2-4R | ACACTCAGAGAGGCGGGCGC | 472 | |
| EVX2-FF | CTGCTGTGTAGCTTCCGCCA | EVX2-R | TATCTGGTGAGCGGAGCCTCGT | 431 | |
| EVX2-FO | CCTACTCGGCCGCGGTGCTTAGTAA | EVX2-O | GGGAAACCAAGCGCTCAGGA | 380 | |
| HOXD13 | |||||
| D13-1F | AGGCTCACAGAGGGAGAGAGGGCTA | D13-1RR | AAGAGGACGACGACGAGGAAGAGCC | 416 | |
| D13-1FF | GCTTTCTCTCCGCGCCTGTGTT | D13-1R | TAAGCCCACGCCGTGCGACATA | 314 | |
| D13-2F | GCTACGGCTACCACTTCGGCAA | D13-2R | TCCCTCGTGTCGGTCCCTTT | 441 | |
| D13-3F | CTAGGTGCTCCGAATATCCCAGC | D13-3R | GGTTGGGCAGCAGAAGGTTT | 480 | |
| HOXD12 | |||||
| D12-1F | CTGCAGGCAAAGTTTCGTCCA | D12-1R | TTGAGAGCAGCCACTGCAGGAG | 471 | |
| D12-2F | CCCAACAGCCAAAGACGGAC | D12-2R | CCGTACTCTGTCTATTGCGCCC | 386 | |
| D12-3F | GGGTTCAAGGGAGAGTGTAAGGG | D12-3R | AGCAGAGAAGGAACCGAAGGAA | 442 | |
| HOXD11 | |||||
| D11-1F | GGCCCAAGCCTCTTGTGCAA | D11-1RR | TAGTCGCGGAAGGCCACTTC | 282 | |
| D11-1FF | CCCGTCTGACTTCGCTAGCA | D11-1R | TGTAGAAGTTGGAGGCGGCG | 425 | |
| D11-2F | GCCATGCAACGCGAGCTTCT | D11-2R | ACGGCCCAGGAAATCTTATAGG | 560 | |
| D11-3F | ACTCTCTCCTGTGCCCGCCCATAT | D11-3R | CGTGAAGTTCCACAGAGAAGAGACG | 507 | |
| HOXD10 | |||||
| D10-1F | TGGCTTGGTTGTCATGTGGTC | D10-1R | GCCGAAATGAGTTTGTTGCG | 490 | |
| D10-2F | CCCACTTGCTCCTTCACCACCA | D10-2R | AGCAGCACCTGCCAAGTCGG | 564 | |
| D10-3F | AGGGTCACGGCCAAGGGTACAT | D10-3R | TTTCCCAGTCCTCCAGCACTG | 511 | |
| D10-3S | CTTCTTCTCCCTTTAATTTTG | D10-3R | TTTCCCAGTCCTCCAGCACTG | 389 | |
| M319k-F | TCAAGATTTGGTTTCAAAACCGCCGC*Aa | D10-3R | TTTCCCAGTCCTCCAGCACTG | 181 | |
| HOXD9 | |||||
| D9-1F | TGTGAGTAGGGAGAGAGGCGAG | D9-1RR | CATAAGCGCCGCAATTAGAGCC | 339 | |
| D9-1FF | GGACCGAGGGCTGCAAGAAGAA | D9-1R | ACATTACACTGTCCGCCGCC | 338 | |
| D9-2F | TGGTAAATATGATCACGTGGGCCG | D9-2R | TAATCCCGTAGTGGCGCCCGTT | 589 | |
| D9-2FF | TCTTCCAGTGGCACCCTCAGCA | D9-3R | TTCGAGCCTTACAGGTTTCAGC | 839 | |
| D9-4F | TCTTCCCCACCCTGTGGTCT | D9-4R | ACCTCAGAGACCAACCACACAGTC | 488 | |
a
The asterisk (*) indicates a nucleotide mismatch that will create an artificial NlaIII restriction recognition site with wild-type but not mutant template.