Table 1.
MAPT H1-SNPs
| Assay Type, ID, SNP, and Primers (5′→3′)a | Alleles | Fragments(bp) | Product(bp) | Enzyme |
| Restriction enzyme digest: | ||||
| 8: | ||||
| rs242937: | ||||
| F: ACCCACAGACCACGACCTTCCAAC | G | 279, 192 | ||
| R: CCTGCCTCTTTCTGCCCATTGG | A | 471 | 471 | MnlI |
| 9: | ||||
| rs242935: | ||||
| F: GGGCCACTGGATCACAAGGTTG | T | 258, 181, 59, 30 | ||
| R: CCGGCCCATAATCTGCATTTCTAAC | C | 288, 181, 59 | 528 | Tsp509I |
| 10: | ||||
| rs242928: | ||||
| F: TTAAGGAAGCACCCATGACAGCC | A | 266, 144, 51 | ||
| R: AAACAGTTCTGTGGAATTTCACCCTG | G | 410, 51 | 461 | MboII |
| 12: | ||||
| rs16339368: | ||||
| F: GGTAGAGGCCAGGAATGCTGTTAAAC | C | 277, 202 | ||
| R: GGTCATGCTCCGATTACAGACTCTTG | A | 479 | 479 | AciI |
| 1: | ||||
| rs242562: | ||||
| F: CAGCCTTCCCTGTCCTTGATTC | G | 385 | ||
| R: GCCTTCCCAACAGAGCAACC | A | 287, 98 | 385 | XhoI |
| 2: | ||||
| rs2435207: | ||||
| F: AGCAAGCTGTGTGACCAG | G | 197, 41 | ||
| R: CCCATTCTCTGACAGATTTG | A | 112, 85, 41 | 238 | BclI |
| 14: | ||||
| rs2258689: | ||||
| F: AGACATCCACACGTTCCTC | C | 132, 107, 9 | ||
| R: CAAACCACAGCAGAGCAG | T | 239, 9 | 248 | AflIII |
| 3: | ||||
| rs16339369: | ||||
| F: CGAGTCCTGGCTTCACTCC | G | 257, 54, 26, 20, 6 | ||
| R: CTTCCAGGCACAGCCATACC | A | 201, 56, 54, 26, 20, 6 | 370 | BstNI |
| 4: | ||||
| rs16339370: | ||||
| F: GGCTGGCCCTGCTCCTTCTCTA | T | 247, 105 | ||
| R: TGGCAAGGACGTTGGGGGACAGGG | C | 352 | 352 | TaiI |
| 5: | ||||
| rs16339371: | ||||
| F: GACTGATAGGTGGGAGGTGGCTGC | CT | 228, 226 | ||
| R: CAGCAGCTCGGACGTGAG | AA | 454 | 454 | PvuII |
| SNaPshot: | ||||
| 6: | ||||
| rs110402: | ||||
| F: GTGCACTCTGTACACTCACTGGACC | C | … | ||
| R: GTATGATTCAGGAATAAGGCAGAAGC | T | … | 483 | … |
| S: CACAGAGGACTGGTGTTGC | … | … | … | … |
| 7: | ||||
| rs171440: | ||||
| F: CTGCACAGAACAAAGTACACGTGAC | G | … | ||
| R: TCCTATGCAAAGAAGACACAAGGG | A | … | 433 | … |
| Sb: aactgaGCGAGGGACCAAGAGAAG | … | … | … | … |
| 11: | ||||
| rs2019820: | ||||
| F: GGTCATCTCTAGTGGGCATTAACACG | C | … | ||
| R: TGACAAAGGCAAGAGTACACAAAGGG | T | … | 381 | … |
| Sb: aactCCAGGCTGTTCTCGAACT | … | … | … | … |
| 13: | ||||
| rs3785883: | ||||
| F: CCATCACCTTGTCAGAAACTC | G | … | ||
| R: AGCCATGTGGTAGCCTCAG | A | … | 277 | … |
| Sb: aactgactaaCACTGTCACCACTGGGC | … | … | … | … |
a
F = forward; R = reverse; S = sequencing.
b
Sequencing primer plus nonannealing sequence.