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. 2005 Aug 1;33(13):4285–4310. doi: 10.1093/nar/gki720

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© The Author 2005. Published by Oxford University Press. All rights reserved

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The complex B4 of the HS7 site B contains PU.1. (A) Nucleotide sequence of the wild-type site B oligonucleotide with the PU box indicated by an arrow on the non-coding strand. The core sequence 5′-GGAA-3′ of the PU box is indicated by a thicker bar. (B) Nuclear extracts from U937 cells (15 μg) were incubated in the absence of antibody (lane 3) or in the presence of a PU.1-specific antiserum (lane 2) or the preimmune rabbit serum as a negative control (lane 1) before the addition of the site B oligonucleotide probe. The same probe was also incubated with whole-cell extracts from SF9 cells infected with an Ac-NVP-PU.1 recombinant baculovirus (PU.1 baculovirus, lane 4). The DNA–protein complexes B1 and B4, the PU.1 complex and the free probe (FP) are indicated by arrows.