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. 2005 Aug 1;33(13):e120. doi: 10.1093/nar/gni122

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Expression and purification of truncated zebrafish Rad52 proteins. (A) SDS–PAGE analysis of bacterially expressed Rad52 1–204Q NLS protein. Ppt. and Sup. are the pellet and supernatant derived from the initial lysate, FT and Elution are the flow-through and eluted fractions of the column fractionations. Rad52 1–204Q NLS showed the predicted molecular weight (MW) of 26.6 kDa. (B) Rad52 1–317Q NLS protein. Lane designations as in (A). The protein migrates as expected for its predicted MW of 39.4 kDa. Mark12; molecular weight marker.