FIG. 1.

Variance within one experiment. DNA of five identical samples, each containing 500 Borrelia equivalents, was extracted with DNAzol. After DNA extraction, one of the five samples was split into five parts, and the resulting nine samples were inserted in the standard PCR. Before real-time PCR was performed, one of the samples was again split into five parts. All 13 samples were quantified in the same real-time PCR run. The means of the split samples are depicted as gray symbols.