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Dilution series of Borrelia DNA in water or urine samples (A and B) from two healthy donors. DNA equivalents of 1,000, 500, 250, 100, 25, and 10 Borrelia organisms were added to 50 μl of water or urine, which should result in concentrations of 10 to 0.1 Borrelia equivalents per μl of eluate. Data are depicted as means ± standard errors of the mean from quadruplicate experiments.
