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Effect of urine centrifugation. A total of 10 ml of urine or water was spiked with 5,000 equivalents of Borrelia DNA (16 different urines) or 5,000 whole Borrelia organisms (11 different urine samples). Either 50 μl of 10 ml spiked urine or water was submitted to DNA extraction (A) or 10 ml urine or water was centrifuged at 40,000 × g for 30 min; the pellet was then submitted to DNA extraction (B).
