TABLE 1.

Antibody staining panels utilized in this study^a

Panel	Target(s) of staining				Expression or cell populations identified
	FITC	PE	PerCP	APC
1	CD14	CD20	CD45	CD38	Major leukocyte populations
2	Lineage cocktail	DC-SIGN and CCR5	HLA DR	CD11c	Activation/maturation markers on DCs
3	Lineage cocktail	CD83, CD83, and CD86	CD86	CD11c	Costimulatory molecules on DCs
4	TCR gamma/delta	CD8	CD3	CD4	Basic T-cell subsets
5	CD45RO	CD27	CD3	CD4	Differentiation of T cells
6	CD69	CD25	CD3	CD4	Activated T cells
7	CD45RO	CD71	CD3	CD4	Early T-cell proliferation marker
8	Lineage cocktail	TLR1, TLR2, or TLR4	HLA DR	CD11c	Expression of TLRs by PBMCs

^aTCR, T-cell receptor; PBMCs, peripheral blood mononuclear cells; APC, antigen-presenting cells.