TABLE 5.
Characterization of T-cell immunophenotypes in PB and BFa
| Surface antigens or phenotype | % Cells with immunophenotype
|
||
|---|---|---|---|
| PB-NL | OspC-1 and 17-lc
|
||
| PB | BF | ||
| CD4/CD8d | |||
| CD3+ CD4+ | 57.0 (48.1-65.9) | 62.7 (56.6-68.9) | 67.7 (61.9-73.5) |
| CD3+ CD8+ | 34.1 (22.7-45.3) | 37.2 (30.9-43.4) | 32.3 (26.5-38.1) |
| CD25/CD69e | |||
| CD4+ CD25+ CD69− | 6.25 (0.8-11.6) | 7.9 (4.5-11.3) | 21.3 (13.9-28.6)b |
| CD4+ CD25− CD69+ | 0.3 (0.04-0.6) | 1.2 (−1.2-3.6) | 13.9 (7.1-20.7)b |
| CD4+ CD25+ CD69+ | 0.02 (0-0.06) | 0.1 (0.00-0.2) | 10.8 (0.5-21.1)b |
| CD4+ CD25− CD69− | 93.4 (88.2-98.6) | 90.5 (88.8-92.2) | 54.0 (37.8-70.2)b |
| CD8+ CD25+ CD69− | 0.5 (0.02-0.99) | 0.8 (−0.1-1.6) | 27.4 (4.5-50.5)b |
| CD8+ CD25− CD69+ | 1.5 (−0.3-3.4) | 0.5 (0.1-0.9) | 3.8 (0.2-7.4) |
| CD8+ CD25+ CD69+ | 0 (0-0) | 0.1 (0.001-0.1) | 6.3 (0.2-7.4) |
| CD8+ CD25− CD69− | 97.9 (96.1-99.8) | 98.7 (97.9-99.5) | 62.4 (35.7-89.0)b |
| CD45RO/CD71f | |||
| CD4+ CD71+ CD45RO− | 0.1 (0-0.2) | 0.2 (−0.2-0.3) | 0 (0-0) |
| CD4+ CD71− CD45RO+ | 51.5 (40.1-52.9) | 30.7 (21.3-40.2) | 77.2 (67.2-87.1)b |
| CD4+ CD71+ CD45RO+ | 0.62 (0.4-0.9) | 0.6 (0.4-0.9) | 10.7 (5.5-15.8)b |
| CD4+ CD71− CD45RO− | 49.46 (36.1-59.2) | 68.3 (59.1-77.6) | 11.5 (4.5-18.5)b |
| CD8+ CD71+ CD45RO− | 0.12 (0-0.3) | 0.1 (0.02-0.2) | 1.1 (0.2-1.9) |
| CD8+ CD71− CD45RO+ | 32.4 (23.7-41.1) | 20.7 (13.9-27.4) | 45.8 (31.5-60.1)b |
| CD8+ CD71+ CD45RO+ | 0.2 (0.1-0.3) | 0.2 (0.1-0.2) | 4.6 (2.4-6.8)b |
| CD8+ CD71− CD45RO− | 67.3 (58.6-76.1) | 79.1 (72.3-85.7) | 48.5 (32.8-64.2)b |
a
Numbers shown are mean percentages with 95% confidence intervals in parentheses. PB-NL, normal (control) blood.
b
Significantly different (P < 0.05), PB versus BF.
c
Each T-cell panel is derived from a combination of subjects injected with either 17-l or OspC-1.
d
For PB-NL, n = 11 samples; for PB, n = 12 samples; for BF, n = 12 samples.
e
For PB-NL, n = 5 samples; for PB, n = 7 samples; for BF, n = 7 samples.
f
For PB-NL, n = 5 samples; for PB, n = 7 samples; for BF, n = 6 samples.