Table 3.

Thermal cycling conditions used in nested PCR assay (B1 gene).

	Step 1-B1	Cycles no	Step 2-B1	Cycles no
Initial denaturation	94ºC 3′		94ºC 3′
Denaturation	94ºC 60′′	30X	94ºC 60′′	35x
Annealing	55ºC 60′′		60ºC 60′′
Extension	72ºC 60′′		72ºC 60′′
Final extension	72ºC 7′		72ºC 7′