FIG. 1.

ICT strip assay results. The test, GST, and control lines were immobilized with GST-NcSAG1t, GST, and mouse anti-GST-NcSAG1t IgG, respectively. GST-NcSAG1t, conjugated with gold colloids, was dried on the conjugate pad. Fifty microliters of serum (1:10 diluted in phosphate-buffered saline) was spotted on the sample pad of a strip. Lane 1, pretest strip; lane 2, negative reaction exhibits no color on the test line; lane 3, positive reaction is indicated by purplish red on the test line; lane 4, nonspecific GST reaction shows purplish red on both test and GST lines. The control line shows color in all the assays.