TABLE 1.
Comparison of ICT with ELISA for detection of antibodies to N. caninum in cattle
| ELISAa results | ICTb test results
|
||
|---|---|---|---|
| No. (%) positive | No. (%) negative | Total (%) | |
| No. (%) positive | 18 (18.8) | 1 (1.0) | 19 (19.8) |
| No. (%) negative | 5 (5.2) | 72 (75.0) | 77 (80.2) |
| Total (%) | 23 (24.0) | 73 (76.0) | 96 (100) |
a
Antibodies to N. caninum were detected by ELISA with GST-NcSAG1t (2). The ELISA was considered positive when an optical density at 415 nm of ≥0.1 was observed at dilutions of 1:100 and above.
b
Antibodies to N. caninum were detected by ICT with GST-NcSAG1t. The ICT was considered positive when both control and test lines turned purplish red at a dilution of 1:10.