Table 2.
Polymorphisms detected in the assembled B. pennsylvanicus genome
Polymorphisms | 497 |
Indels | 52 |
Average size, bp | 1.58 |
Intergenic regions | 50 |
Pseudogenes | 0 |
RNAs | 0 |
ORFs | 2 |
SNPs | 445 |
Transitions | 331 |
Transversions | 114 |
Ratio | 2.9 |
Intergenic Spacers | 156 |
Pseudogenes | 5 |
RNAs | 1 |
ORFs | 283 |
Synonymous | 176 |
1st | 7 |
2nd | 1 |
3rd | 168 |
Nonsynonymous | 107 |
1st | 66 |
2nd | 37 |
3rd | 4 |
Nucleotide polymorphisms were recorded for all sites in the genome where high quality (>40 PHRED score) disagreements occurred, and where both variants were represented by multiple clones (at least two clones, and typically many more). The two indels within ORFs did not disrupt the reading frame, but rather resulted in amino acid insertions or deletions, that is, insertion/deletion of a leucine and lysine in sucA (sites 348-349 of the protein sequence) and the insertion/deletion of an alanine in aroE (between sites 126 and 127 of the protein sequence).