Figure 3.

Highly conserved non-coding regions (HCNRs) located between Irx3 and Irx5 genes show enhancer activity in Xenopus. Lateral views of late neurula Xenopus embryos showing enhanced green fluorescent protein mRNA localization promoted by different HCNRs (A–F). The number in the lower righthand corner of each panel corresponds to the position of the human homologous region in chromosome 16 (NCBI build 33) as shown in Figure 1I. Illustrations show a schematic representation of the expression domains in which the HCNRs are active. (fb) Forebrain; (mb) midbrain; (hb) hindbrain; (ep) epidermis; (ey) eye; (p) pronephros.