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. 2005 Jul 7;24(15):2730–2740. doi: 10.1038/sj.emboj.7600747

Figure 3.

Figure 3

Constitutive activity of SplitΔRpIND and restoration of PKA regulation by R domain. Iodide effluxes were measured from BHK cell monolayers at 1 min intervals. (A) Initial effluxes from BHK cells stably transfected with SplitΔRpIND with or without induction by 10 μM Ponasterone A for 48 h. Initial iodide effluxes from cells constitutively expressing full-length wild-type (WT) CFTR are shown for comparison. (B) Effect of the cAMP agonists 150 μM cpt-cAMP+1 mM IBMX added at t=0 min on iodide efflux from cells induced to express SplitΔRpIND or SplitΔRpIND+RDpNUT. Control cells were not induced. (C) Effect of R domain coexpression on basal halide permeability assayed as initial iodide efflux. (D) Restoration of iodide efflux response to cAMP agonists when R domain is coexpressed with SplitΔR CFTR. Means±s.e., n=6.