FIGURE 5.

Arginine metabolism in fresh and stored murine RBCs (n = 525 animals). (a) Experimental overview of the generation of diversity outbred mice whose RBCs were characterized by multi‐omics approaches at Days 0 and 7 of storage. (b and c) Metabolite correlations (Spearman) to arginine levels in fresh (b) and stored (c) RBCs. Arginine pathway metabolites are highlighted in red. (d–g) Manhattan plots of metabolite–SNP associations in fresh RBCs from mice, highlighting arginine (d), ornithine (e), citrulline (f), and spermine (g). (h and i) Volcano plot illustrating the effects of sex on levels of arginine pathway metabolites (shown in red) in fresh (h) and stored (i) mouse RBCs. Blue shaded region indicates arginine metabolites increased in RBCs from males versus females; light red shaded region indicates arginine metabolites increased in RBCs from females versus males.