Figure 3.

Localization of Bni4, Cdc11, and Cdc12 by indirect immunofluorescence. Unbudded cells of strain YLK281 were incubated in Lat-A-containing medium as described in Figure 2D. (A and B) Bni4 (A), Cdc11 (B), and Cdc12 (B) were visualized as rings or, more frequently, as caps. (C) Samples stained with anti-Cdc11 antibody were analyzed using a confocal microscope (see Materials and Methods), by using a Z-section scan to create a 3D projection image. One and 2 show an image of one focal plane and a corresponding 90° angle projection image, respectively, of a septin ring. Three to 6 show similar pairs of images of cells for which the Cdc11 signal was a cap. See also the supplementary videos showing 3D projections of a ring and caps (Fig 3c1.mov, Fig 3c2.mov, and Fig 3c3.mov). Bars, 1 μm.