Figure 4.

Septin caps can rearrange into rings when F-actin is restored. Unbudded cells of CDC10-GFP strain YLK66 were incubated in medium containing 150 μM Lat-A for 4 h and washed into Lat-A-free medium. (A) A cell that budded in a location previously marked by a cap (arrow). (B) A cell that budded in a location distant from the cap, whose signal diminished during the incubation (arrows). (C–E) Images in one focal plane (1) and the corresponding 90° angle projection images (2) of a control cell treated with DMSO (C) and of cells that were treated with Lat-A for 4 h (D and E). (E, 3) A 90° angle projection image of the same cells in 1 and 2 after 2 h of incubation on Lat-A-free medium. C-E are accompanied by supplemental video materials (Fig 4c.mov, Fig 4d.mov, Fig 4e1.mov, and Fig 4e2.mov). Bars, 1 μm.