Figure 6.

Bni4 and Kcc4 localization in gin4Δ and elm1Δ mutants. DIC, fluorescence, and merged images are shown. (A) A gin4Δ strain expressing Cdc10-GFP (YLK158) was grown at 37°C. Cdc10-GFP was often found diffusely throughout most of the bud plasma membrane (2, arrow) or as bars in the neck (2 and 4, arrowheads). In some cells with elongated buds, septins were detected both at the neck and at the tip of the bud (4, arrows). (B and C) A gin4Δ strain expressing Bni4-CFP and Kcc4-YFP (YLK202) was grown either at 24°C (B, 1–4) or for 5 h at 37°C (B, 5–8, and C). (D) An elm1Δ strain expressing Bni4-CFP and Kcc4-YFP (JRL49) was grown at 37°C. Green, Bni4-CFP; red, Kcc4-YFP. Bars, 1 μm.