Figure 7.

Bni4 and Kcc4 localization in cdc3 and shs1 mutants. DIC, fluorescence, and merged images are shown. (A and B) cdc3-1 cells expressing Bni4-CFP and Kcc4-YFP (YLK271) were imaged after a 2 h incubation at 30°C (A, 1–4), a 3-h incubation at 30°C (A, 5–8), or a 6-h incubation at 28°C (B, 1–6). (C and D) shs1Δ cells expressing Bni4-YFP and Kcc4-CFP (JRL18) were imaged after a 3-h incubation at 37°C (C) or after a 2.5-h incubation at 30°C (D). Green, Bni4-CFP; red, Kcc4-YFP. Bars, 1 μm. Arrows indicate structures described in the text.