Table 2.

Effect of Lat-A treatment on the spatial organization of Kcc4 and Bni4

	DMSO	Lat-A
	3.5 h (n = 648)	3.5 h (n = 1779)	4.25 h (n = 948)	8 h (n = 875)
% Unbudded cells	54	96	97	95
No. of unbudded cells with a ring	16	6	1	0
Diameter of Kcc4-YFP ring (μm)	0.92 ± 0.09	1.02 ± 0.21	1.08
Diameter of Bni4-CFP ring (μm)	1.15 ± 0.09	1.46 ± 0.19	1.20
Kcc4-YFP/Bni4-CFP ring ratio	0.80 ± 0.09	0.70 ± 0.07	0.90
No. of unbudded cells with a cap	3	39	25	54
Diameter of Kcc4-YFP cap (μm)	0.75 ± 0.07	1.06 ± 0.25	1.22 ± 0.25	1.15 ± 0.28
Diameter of Bni4-CFP cap (μm)	0.99 ± 0.17	1.14 ± 0.22	1.20 ± 0.21	1.18 ± 0.28
Kcc4-YFP/Bni4-CFP cap ratio	0.77 ± 0.06	0.93 ± 0.14	1.02 ± 0.60	0.99 ± 0.15
No. of unbudded cells with an indeterminate signala	0	6	4	4
Diameter of Kcc4 indeterminate signal (μm)		1.11 ± 0.17	1.26 ± 0.25	1.28 ± 0.09
Diameter of Bni4 indeterminate signal (μm)		1.28 ± 0.17	1.28 ± 0.05	1.29 ± 0.17
Kcc4/Bni4 diameter ratio for indeterminate signal		0.87 ± 0.13	0.99 ± 0.21	1.00 ± 0.07
% of Unbudded cells with lost asymmetryb	5	49	77	74

Unbudded cells of strain YLK189 were isolated and treated with 150 μM Lat-A as described in Materials and Methods. Only cells with both proteins visible were scored.

^aThe Kcc4 and Bni4 signals were intermediate between rings and caps.

^bLost asymmetry is defined as a ratio of Kcc4 to Bni4 signal diameter of ≥0.9.