Table 3.

Effect of Lat-A on the localization of Bni4 and septins

Line	Strain	Signal	Concentration of Lat-A (μM)	% of Unbudded cells with signal	% of Signals detected as ringsa	No. of cells scored
1	YLK281	Bni4-GFP	100	34	24	395
2	YLK281	Cdc3-GFP	100	70	26	435
3	KT1357	anti-Bni4	100	9	44	1272
4	YLK281	anti-Bni4	100	12	33	1721
5	YLK281	anti-Cdc11	100	37	19	288
6	YLK281	anti-Cdc11	200	37	27	386
7	YLK281	anti-Cdc11	400	35	29	375
8	YLK281	anti-Cdc12	100	39	33	554
9	YLK281	anti-Cdc12	200	43	30	419
10	YLK281	anti-Cdc12	400	43	28	335

Unbudded cells of strains YLK281 (S288C background) and KT1357 (JC482 background) were isolated, treated with Lat-A, and prepared for indirect immunofluorescence as described in Materials and Methods. Bni4-GFP (from plasmid pLK10) and Cdc3-GFP (from plasmid pTD150-CDC3) were imaged in live cells.

^aThe remaining signals in each case seemed to be caps.